Detection of yeast strains by combining surface-imprinted polymers with impedance-based readout

In this work, we present a new technique for the sensitive and facile detection of Saccharomyces strains with limit 30 cells/mL in both buffer solution food samples such as beer yoghurt. This is achieved by combining ultrathin surface-imprinted polymer (SIP) layers receptors impedance spectroscopy readout principle. Binding cells to imprinted surface results concentration-dependent increase signal. According equivalent-circuit modelling spectra, due an resistive component solid-liquid interface while capacitive effects are negligible. Regarding selectivity, prepared imprints three different template compared sensor response using same targets. The strongest combinations which templates targets belong strain. For other situations, signal reduced factor three, indicating certain level can be interpreted terms size cell wall differences. summary, combination SIP-type offers fast on-site analysis complex matrices outside laboratory environment. yeast should transferable microorganisms, including pathogenic ones.